PEI-Mediated Transient Mammalian Expression Services

Kempbio, Inc. has over 15 years of experience with the transient expression of recombinant proteins in suspension cultures of mammalian host cells at the 1-liter to 100-liter scale. We produce our own proprietary formulation of PEI which allows us to offer exceptional expression results at a reasonable cost. Transfections utilize either HEK-293 or CHO-S cells as the host cell for expression and both systems are scalable from 1-liter to 100 liters and above.

Typical Services Provided:

  • Gene Synthesis
  • Low Endotoxin Plasmid Production and Purification (1-100 mg)
  • Expression Optimization
  • HEK-293 or CHO-S Host Cells
  • Serum-free Expression
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

Proteins Expressed and Purified:

  • Monoclonal Antibodies
  • Viral Glycoproteins (filoviral)
  • Influenza VLPs
  • Protease Inhibitors
  • Membrane Proteins

Example:

HEK-293 Cells Transfected Using PEI/DNA Complex: Viral Glycoprotein

PEI-Mediated-Transient

Expression and purification of a secreted viral glycoprotein. Lane 3 represents the load for an affinity column and Lane 4 is the column flow-through fraction. Lanes 5 through 10 are elution fractions.

 

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