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Mammalian Expression Services

The Kemp Proteins team has more than two decades of experience and technical expertise with transient and stable expression of recombinant proteins in a variety of mammalian host cells. We have successfully delivered a range of customer-defined proteins, including hundreds of difficult-to-express, novel proteins (never previously expressed) that have required our scientists to “think outside the box.” This breadth of experience in mammalian expression makes our team the choice in the industry for expressing your protein.

We offer a complete gene-to-protein process using our proprietary formulation of PEI that allows us to offer exceptional expression results at a reasonable cost. In addition, we offer “a la carte” mammalian protein expression services, including test expression, production, purification, and characterization of recombinant mammalian clones as well as process development and scale-up for protein expression.

Our production service includes serum-free, multi-liter-scale that provides post-purification products with low endotoxin levels suitable for animal studies.

We are flexible in our approach to working with you, we provide bi-weekly updates, and at the end of every project, we can provide comprehensive documentation to assist with transfer to GMP production.

Use our quick quote form to request a quote.

Not sure which cell line to use? Get in touch.

The Details:

Mammalian Cell Expression Platforms:
  • CHO
  • CHO-S
  • CHO-DG44
  • CHO-SSI
  • HEK-293
  • Hybridoma cell line
  • NS0

Our service offerings include:
  • Gene to Protein
  • Low Endotoxin Plasmid Production and Purification (1–100 mg)
  • Expression Optimization
  • Transient Expression in HEK-293 or CHO-S Host Cells
  • Serum-Free Expression
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

We express and purify the following proteins:
  • Monoclonal antibodies
  • Antibody fragments
  • Bi-specific antibodies
  • VHH
  • Viral glycoproteins
  • Virus-like particles (VLPs)
  • Multi-protein complexes
  • Nanoparticles
  • Proteases
  • Membrane proteins / GCPRs (no purification)
  • Extracellular domains (ECDs)
  • Enzymes
  • Protein hormones

Workflow & Timeline: Transient Mammalian Expression

Kemp Proteins Transient Mammalian Expression Workflow & Timeline

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