BacMam Expression Services

BacMam recombinant baculoviruses are easily amplified in insect cells and efficiently transduce a wide variety of mammalian cell lines. We have developed a robust and scalable protein expression platform based on the BacMam system. We currently offer a complete line of services including production, purification and characterization of recombinant BacMam baculovirus clones in addition to process development and scale-up for protein expression. Culture volumes from 1 L to 100 liters are available using serum-free media formulations and HEK-293 or CHO-S host cell lines. Optimization of protein expression is accomplished using titration experiments to determine the optimal virus to cell infection ratio and time of harvest. Cell lysate production, supernatant concentration and protein purification services are available.

Typical Services Provided:

  • Gene Synthesis
  • Virus production
  • Clone Isolation and Screening
  • High-Titer Stocks
  • Virus Banking
  • Time Course/MOI
  • Virus Production and Characterization
  • Bioreactor Volumes from 2-L to 100-L
  • HEK-293 and CHO-S Host Cell Lines

Proteins Expressed and Purified:

  • Monoclonal Antibodies
  • Viral Glycoproteins
  • Membrane Proteins
  • Virus-like Particles (VLPs)
  • Enzymes

Example:

BacMam transduced HEK-293 cells produce >150 mg/liter purified human IgG1 when harvested 72 hours post-transfection.

BacMam

Example:

BacMam mediated transient expression of Human rIgG1 in serum-free suspension cultures of CHO-S cells. Reported yields are post-purification over MabSelect Sure. The figure and graph illustrate the relationship between cell density, virus concentration and productivity.

CellDensity

Example:

Expression of H7N1 influenza VLPs from HEK-293 cells co-transduced with three BacMam viruses.

H7N1

 

 

Contact Us

Tell us about your project and we will reply shortly.